necrotic effect versus apoptotic nature of camptothecin in human cervical cancer cells

نویسندگان

abbas zare mirakabadi dept. of venomous animals and antivenom production, razi vaccine and serum research institute, karaj, iran

ali sarzaeem dept. of venomous animals and antivenom production, razi vaccine and serum research institute, karaj, iran dept. of biology, payam-e-noor university, tehran, iran

saeed moradhaseli dept. of venomous animals and antivenom production, razi vaccine and serum research institute, karaj, iran

aida sayad dept. of biochemistry, faculty of medicine, tarbiat modares university, tehran, iran

چکیده

background: functional defects in mitochondria are involved in the induction of cell death in cancer cells. the process of programmed cell death may occur through the mechanisms of apoptosis. several potential lead molecules such as camptothecin (cpt) and its analogues have been isolated from plants with anticancer effect. the aim of the present study was to understand the necrotic effect versus apoptotic nature of cpt in hela cancer cells. methods: the antiproliferative activity of cpt was estimated through 3-(4, 5- dimethyl thiazol-2-yl)-2, 5-diphenyl tetrazolium bromide (mtt) assay and dna fragmentation analysis using gel electrophoresis. lactate dehydrogenase (ldh) activity and cell morphology were assessed under control and cpt exposed conditions to evaluate the necrotic effect of cpt. results: the results showed that cpt inhibited the proliferation of hela cells in a dose-dependent manner with an inhibitory concentration 50% (ic 50 ) of 0.08±0.012 µg/ml. however the significant (p<0.05) increase happens in ldh activity at concentrations 1×10 -1 µg/ml and above. morphological changes showed that cpt in low concentrations induced an apoptotic mechanism of cell death, such as cell shrinkage and characteristic rounding of dying cells, while at high concentrations showed necrosis changes. the characteristic dna ladder formation of cpt-treated cells in agarose gel electrophoresis confirmed the results obtained by light microscopy and ldh assay. conclusion: camptothecin as an anticancer drug may have antiproliferative effect on hela cancer cells in low concentrations, through its nature of induction of apoptosis. the border line between necrotic effect and apoptotic nature of cpt in hela cancer cells has been found to be at concentration of 1×10 -1 µg/ml.

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عنوان ژورنال:
iranian journal of cancer prevention

جلد ۵، شماره ۳، صفحات ۱۰۹-۱۱۶

کلمات کلیدی
[ ' c e l l d e a t h ' , ' c a m p t o t h e c i n ' , ' h e l a c e l l s ' , ' n e c r o s i s ' , ' a p o p t o s i s p l e a s e c i t e t h i s a r t i c l e a s ' , ' z a r e m i r a k a b a d i a ' , ' s a r z a e e m a ' , ' m o r a d h a s e l i s ' , ' s a y a d a ' , ' n e g a h d a r y m . n e c r o t i c e f f e c t v e r s u s a p o p t o t i c n a t u r e o f c a m p t o t h e c i n i n h u m a n c e r v i c a l c a n c e r c e l l s . i r a n j c a n c e r p r e v . 2 0 1 2 ' , ' 5 ( 3 ) ' , 1 0 9 , ' 1 6 . r e f e r e n c e s 1 . b r a s m ' , ' q u e e n a n b ' , ' s u s i n s a . p r o g r a m m e d c e l l d e a t h v i a m i t o c h o n d r i a ' , ' d i f f e r e n t m o d e s o f d y i n g . b i o c h e m i s t r y ( m o s c ) . 2 0 0 5 ' , 7 0 , 2 3 1 , ' 9 . 2 . k r o e m e r g ' , ' g a l l u z z i l ' , ' v a n d e n a b e e l e p ' , ' a b r a m s j ' , ' a l n e m r i e s ' , ' b a e h r e c k e e h ' , ' e t a l . c l a s s i f i c a t i o n o f c e l l d e a t h ' , ' r e c o m m e n d a t i o n s o f t h e n o m e n c l a t u r e c o m m i t t e e o n c e l l d e a t h . c e l l d e a t h d i f f e r . 2 0 0 9 ' , 1 6 , 3 , ' 1 1 . 3 . s c h w a r t z m a n r a ' , ' c i d l o w s k i j a . a p o p t o s i s ' , ' t h e b i o c h e m i s t r y a n d m o l e c u l a r b i o l o g y o f p r o g r a m m e d c e l l d e a t h . e n d o c r i n e r e v . 1 9 9 3 ' , 1 4 , ' 1 3 3 . 4 . v e r m e s i ' , ' h a a n a n c . a p o p t o s i s a n d p r o g r a m m e d c e l l d e a t h i n h e a l t h a n d d i s e a s e . a d v c l i n c h e m . 1 9 9 4 ' , 3 1 , ' 1 7 7 . 5 . s a n g w h ' , ' y u n j k ' , ' w o n y o n g k ' , ' c h u n g s l . a n t i t u m o r e f f e c t s o f c a m p t o t h e c i n c o m b i n e d w i t h c o n v e n t i o n a l a n t i c a n c e r d r u g s o n t h e c e r v i c a l a n d u t e r i n e s q u a m o u s c e l l c a r c i n o m a c e l l l i n e s i h a . k o r e a n j p h y s i o l p h a r m a c o l . 2 0 0 9 ' , 1 3 , 1 1 5 , ' 2 1 . 6 . k e r r j f ' , ' w y l l i e a h ' , ' c u r r i e a r . a p o p t o s i s ' , ' a b a s i c b i o l o g i c a l p h e n o m e n o n w i t h w i d e ' , ' r a n g i n g i m p l i c a t i o n s i n t i s s u e k i n e t i c s . b r j c a n c e r . 1 9 7 2 ' , 2 6 , 2 3 9 , ' 5 7 . 7 . w y l l i e a h ' , ' k e r r j f ' , ' c u r r i e a r . c e l l d e a t h ' , ' t h e s i g n i f i c a n c e o f a p o p t o s i s . i n t r e v c y t o l . 1 9 8 0 ' , 6 8 , 2 5 1 , ' 3 0 6 . 8 . w y l l i e a h . a p o p t o s i s ( t h e 1 9 9 2 f r a n k r o s e m e m o r i a l l e c t u r e ) . b r j c a n c e r . 1 9 9 3 f e b ' , 6 7 , 2 0 5 , ' 8 . 9 . m a j n o g ' , ' j o r i s i . a p o p t o s i s ' , ' o n c o s i s ' , ' a n d n e c r o s i s . a n o v e r v i e w o f c e l l d e a t h . a m j p a t h o l . 1 9 9 5 ' , 1 4 6 , ' 3 . 1 0 . r u d i n c m ' , ' t h o m p s o n c b . a p o p t o s i s a n d d i s e a s e ' , ' r e g u l a t i o n a n d c l i n i c a l r e l e v a n c e o f p r o g r a m m e d c e l l d e a t h . a n n r e v m e d . 1 9 9 7 ' , 4 8 , 2 6 7 , ' 8 1 . 1 1 . f i e r s w ' , ' b e y a e r t r ' , ' d e c l e r c q w ' , ' v a n d e n a b e e l e p . m o r e t h a n o n e w a y t o d i e ' , ' a p o p t o s i s ' , ' n e c r o s i s a n d r e a c t i v e o x y g e n d a m a g e . o n c o g e n e . 1 9 9 9 ' , 1 8 , 7 7 1 9 , ' 3 0 . 1 2 . s i l v a r j ' , ' s i l v a m g ' , ' v i l e l a l c ' , ' f e c c h i o d . a n t i t u m o r e f f e c t o f b o t h r o p s j a r a r a c a v e n o m . m e d i a t o r s i n f l a m m . 2 0 0 2 ' , 1 1 , 9 9 , ' 1 0 4 . 1 3 . s o n d j ' , ' p a r k m h ' , ' c h a e s j ' , ' m o o n s o ' , ' l e e j w ' , ' s o n g h s ' , ' e t a l . i n h i b i t o r y e f f e c t o f s n a k e v e n o m t o x i n f r o m v i p e r a l e b e t i n a t u r a n i c a o n h o r m o n e ' , ' r e f r a c t o r y h u m a n p r o s t a t e c a n c e r c e l l g r o w t h ' , ' i n d u c t i o n o f a p o p t o s i s t h r o u g h i n a c t i v a t i o n o f n u c l e a r f a c t o r b . m o l c a n c e r t h e r a p e u t i c s . 2 0 0 7 ' , 6 , 6 7 5 , ' 8 3 . 1 4 . y a n g r s ' , ' t a n g c h ' , ' c h u a n g w j ' , ' h u a n g t h ' , ' p e n g h c ' , ' h u a n g t f ' , ' e t a l . i n h i b i t i o n o f t u m o r f o r m a t i o n b y s n a k e v e n o m d i s i n t e g r i n . t o x i c o n . 2 0 0 5 ' , 4 5 , 6 6 1 , ' 9 . 1 5 . t s a o y p ' , ' r u s s o a ' , ' n y a m u s w a g ' , ' s i l b e r r ' , ' l i u l f . i n t e r a c t i o n b e t w e e n r e p l i c a t i o n f o r k s a n d t o p o i s o m e r a s e ' , ' i ' , ' d n a c l e a v a b l e c o m p l e x e s ' , ' s t u d i e s i n a c e l l ' , ' f r e e s v 4 0 d n a r e p l i c a t i o n s y s t e m . c a n c e r r e s . 1 9 9 3 ' , 5 3 , 5 9 0 8 , ' 9 1 4 . 1 6 . l a c o g s ' , ' c o l l i n s j r ' , ' l u k e b t ' , ' k r o t h h ' , ' s a y e r j m ' , ' j e r i n a d m ' , ' e t a l . h u m a n t o p o i s o m e r a s e i i n h i b i t i o n ' , ' d o c k i n g c a m p t o t h e c i n a n d d e r i v a t i v e s i n t o a s t r u c t u r e ' , ' b a s e d a c t i v e s i t e m o d e l . b i o c h e m i s t r y . 2 0 0 2 ' , 4 1 , 1 4 2 8 , ' 3 5 . 1 7 . s r i v a s t a v a v ' , ' a r v i n d s n ' , ' k u m a r j k ' , ' g u p t a m m ' , ' s u m a n p s . p l a n t ' , ' b a s e d a n t i c a n c e r m o l e c u l e s ' , ' a c h e m i c a l a n d b i o l o g i c a l p r o f i l e o f s o m e i m p o r t a n t l e a d s . b i o o r g a n i c & ' , ' m e d i c i n a l c h e m i s t r y . 2 0 0 5 ' , 1 3 , 5 8 9 2 , ' 9 0 8 . 1 8 . w a l l m e ' , ' w a n i m c ' , ' c o o k c e ' , ' p a l m e r k h ' , ' m c p h a i l a t ' , ' s i m g a . p l a n t a n t i t u m o u r a g e n t s . i . t h e i s o l a t i o n a n d s t r u c t u r e o f c a m p t o t h e c i n ' , ' a n o v e l a l k a l o i d a l l e u k e m i a a n d t u m o u r i n h i b i t o r f r o m c a m p t o t h e c a a c u m i n a t a . j a m c h e m s o c . 1 9 6 6 ' , 8 8 , 3 8 8 8 , ' 9 0 . 1 9 . w a l l m e . c a m p t o t h e c i n a n d t a x o l ' , ' d i s c o v e r y t o c l i n i c . m e d r e s r e v . 1 9 9 8 ' , 1 8 , 2 9 9 , ' 3 1 4 . 2 0 . h o r w i t z s b ' , ' c h a n g c ' , ' g r o l l m a n a p . a n t i v i r a l a c t i o n o f c a m p t o t h e c i n . a n t i m i c r o b i a l a g e n t s a n d c h e m o t h e r a p y . 1 9 7 2 ' , 2 , 3 9 5 , ' 4 0 1 . 2 1 . r e d i n b o m r ' , ' s t e w a r t l ' , ' k u h n p ' , ' c h a m p o u x j j ' , ' h o l w g j . c r y s t a l s t r u c t u r e s o f h u m a n t o p o i s o m e r a s e i i n c o v a l e n t a n d n o n ' , ' c o v a l e n t c o m p l e x e s w i t h d n a . s c i e n c e . 1 9 9 8 ' , 2 7 9 , 1 5 0 4 , ' 5 1 3 . 2 2 . s t a k e r b l ' , ' h j e r r i l d k ' , ' f e e s e m d ' , ' b e h n k e c a ' , ' b u r g i n a b ' , ' s t e w a r t l p . t h e m e c h a n i s m o f t o p o i s o m e r a s e i p o i s o n i n g b y a c a m p t o t h e c i n a n a l o g . n a t l a c a d s c i u . s . a . 2 0 0 2 ' , 9 9 , 1 5 3 8 7 , ' 9 2 . 2 3 . h o r w i t z s b ' , ' c h a n g c k ' , ' g r o l l m a n a p . s t u d i e s o n c a m p t o t h e c i n ' , ' e f f e c t s o n n u c l e i c a c i d a n d p r o t e i n s y n t h e s i s . m o l p h a r m a c o l . 1 9 7 1 ' , 7 , 6 3 2 , ' 4 4 . 2 4 . k e s s e l d . e f f e c t s o f c a m p t o t h e c i n o n r n a s y n t h e s i s i n l e u k e m i a l 1 2 1 0 c e l l s . b i o c h i m b i o p h y s a c t a . 1 9 7 1 ' , 2 4 6 , 2 2 5 , ' 3 2 . 2 5 . k a s s e l d . s o m e d e t e r m i n a n t s o f c a m p t o t h e c i n r e s p o n s i v e n e s s i n l e u k e m i a l 1 2 1 0 c e l l s . c a n c e r r e s . 1 9 7 1 ' , 3 1 , 1 8 8 3 , ' 7 . 2 6 . l i c j ' , ' w a n g c ' , ' p a r d e e a b . i n d u c t i o n o f a p o p t o s i s b y β ' , ' l a p a c h o n e i n h u m a n p r o s t a t e c a n c e r c e l l s . c a n c e r r e s . 1 9 9 5 ' , 5 5 , 3 7 1 2 , ' 5 . 2 7 . n a r d u . t h e p r o a p o p t o t i c d r u g c a m p t o t h e c i n s t i m u l a t e s p h o s p h o l i p a s e d a c t i v i t y a n d d i a c y l g l y c e r o l p r o d u c t i o n i n t h e n u c l e u s o f h l ' , ' 6 0 h u m a n p r o m y e l o c y t i c l e u k e m i a c e l l s . c a n c e r r e s . 1 9 9 9 ' , 5 9 , 3 9 6 1 , ' 7 . 2 8 . n i e v e s n w ' , ' p o m m i e y . a p o p t o t i c r e s p o n s e t o c a m p t o t h e c i n a n d 7 ' , ' h y d r o x y s t a u r o s p o r i n e ( u c n ' , ' 0 1 ) i n t h e 8 h u m a n b r e a s t c a n c e r c e l l l i n e s o f t h e n c i a n t i c a n c e r d r u g s c r e e n ' , ' m u l t i f a c t o r i a l r e l a t i o n s h i p s w i t h t o p o i s o m e r a s e i ' , ' p r o t e i n k i n a s e c ' , ' b c l ' , 2 , ' p 5 3 ' , ' m d m ' , ' 2 a n d c a s p a s e p a t h w a y s . i n t j c a n c e r . 1 9 9 9 ' , 8 2 , 3 9 6 , ' 4 0 4 . 2 9 . o h y a m a t ' , ' l i y ' , ' u t s u g i t ' , ' i r i e s ' , ' y a m a d a y ' , ' s a t o t . a d u a l t o p o i s o m e r a s e i n h i b i t o r ' , ' t a s ' , 1 0 3 , ' i n d u c e s a p o p t o s i s i n h u m a n c a n c e r c e l l s . j p n j c a n c e r r e s . 1 9 9 9 ' , 9 0 , 6 9 1 , ' 8 . 3 0 . y o u n g n a m c ' , ' r i y i s ' , ' r i c h a r d b b ' , ' a l b e n a i . f u n c t i o n a l i z e d m e s o p o r o u s s i l i c a n a n o p a r t i c l e ' , ' b a s e d d r u g d e l i v e r y s y s t e m t o r e s c u e a c r o l e i n ' , ' m e d i a t e d c e l l d e a t h . n a n o m e d i c i n e . 2 0 0 8 ' , 3 , 1 , ' 1 3 . 3 1 . p o u r h a s s a n m ' , ' z a r g h a m i n ' , ' r a h m a t i m ' , ' a l i b a k h s h i ' , ' r a n j b a r i j . t h e i n h i b i t o r y e f f e c t o f c u r c u m a l o n g a e x t r a c t o n t e l o m e r a s e a c t i v i t y i n a 5 4 9 l u n g c a n c e r c e l l l i n e . a f r i c a n j o u r n a l o f b i o t e c h n o l o g . 2 0 1 0 ' , 9 , 9 1 2 , ' 1 9 . 3 2 . f i o n a m y ' , ' w i c h a y a p ' , ' b a r b a r a j s s . m o d i f i c a t i o n o f m t t a s s a y c o n d i t i o n s t o e x a m i n e t h e c y t o t o x i c e f f e c t s o f a m i t r a z o n t h e h u m a n l y m p h o b l a s t o i d c e l l l i n e ' , ' w i l 2 n s . t o x i c o l i n v i t r o . 2 0 0 5 ' , 1 9 , 1 0 5 1 , ' 9 . 3 3 . h a r b e l l j w ' , ' k o o n t z s w ' , ' l e w i s r w ' , ' l o v e l l d . a c o s t a c e l l c y t o t o x i c i t y a s s a y s . f o o d c h e m t o x i c o l . 1 9 9 7 ' , 3 5 , 7 9 , ' 1 2 6 . 3 4 . h u s o y t ' , ' s y v e r s e n t ' , ' j e n s s e n j . c o m p a r i s o n s o f f o u r i n v i t r o c y t o t o x i c i t y t e s t s ' , ' t h e m t t a s s a y ' , ' n r a s s a y ' , ' u r i d i n e i n c o r p o r a t i o n a n d p r o t e i n m e a s u r e m e n t s . t o x i c o l i n v i t r o . 1 9 9 3 ' , 7 , 1 4 9 , ' 5 4 . 3 5 . j a m e s f d o ' , ' w i l l i a m c b ' , ' m a r t i n g ' , ' k e n t c ' , ' u l l a c ' , ' s t e v e n p m ' , ' e t a l . c o m p a r i s o n o f c i s p l a t i n a n d c a r b o p l a t i n c y t o t o x i c i t y i n h u m a n o v a r i a n c a n c e r c e l l l i n e s u s i n g t h e m t t a s s a y . g y n e c o l o n c o l . 1 9 9 0 ' , 3 9 , 1 1 9 , ' 2 2 . 3 6 . m o s m a n n t . r a p i d c o l o r i m e t r i c a s s a y f o r c e l l u l a r g r o w t h a n d s u r v i v a l ' , ' a p p l i c a t i o n t o p r o l i f e r a t i o n a n d c y t o t o x i c i t y a s s a y s . j i m m u n o l m e t h o d s . 1 9 8 3 ' , 6 5 , 5 5 , ' 6 3 . 3 7 . s u b h a d r a d e v i v ' , ' k a l a t h i l k ' , ' k u p p u s a m y a ' , ' m u t h u s w a m y u a s ' , ' p u l i y a t h j . i n d u c t i o n o f a p o p t o s i s a n d c y t o t o x i c a c t i v i t i e s o f a p i u m g r a v e o l e n s l i n n . u s i n g i n v i t r o m o d e l s . m i d d l e ' , ' e a s t j o u r n a l o f s c i e n t i f i c r e s e a r c h . 2 0 1 1 ' , 9 , 9 0 , ' 9 4 . 3 8 . c h e n g b i n x ' , ' w e i l ' , ' j i a n h o n g w ' , ' x i a n g l i a n g y ' , ' h u i b i x . c h e m o p r o t e c t i v e e f f e c t o f n ' , ' a c e t y l c y s t e i n e ( n a c ) o n c e l l u l a r o x i d a t i v e d a m a g e s a n d a p o p t o s i s i n d u c e d b y n a n o t i t a n i u m d i o x i d e u n d e r u v a i r r a d i a t i o n . t o x i c o l o g y i n v i t r o . 2 0 1 1 ' , 2 5 , 1 1 0 , ' 1 6 . 3 9 . j o s é m c ' , ' j u d i t c m ' , ' e l e n a p ' , ' g e r a r d o a c ' , ' m a r i a a p ' , ' m a n u e l a p . t h e a n t i m i c r o b i a l p e p t i d e c e c r o p i n a i n d u c e s c a s p a s e ' , ' i n d e p e n d e n t c e l l d e a t h i n h u m a n p r o m y e l o c y t i c l e u k e m i a c e l l s . p e p t i d e s . 2 0 1 0 ' , 3 1 , 1 4 9 4 , ' 5 0 3 . 4 0 . s a e e d s ' , ' m o h a m m a d h b ' , ' s a i d e h d . u s e o f i n v i t r o a s s a y s t o a s s e s s t h e p o t e n t i a l a n t i p r o l i f e r a t i v e a n d c y t o t o x i c e f f e c t s o f s a f f r o n ( c r o c u s s a t i v u s l . ) i n h u m a n l u n g c a n c e r c e l l l i n e . p h a r m a c o g n m a g . 2 0 1 0 ' , 6 , 3 0 9 , ' 3 1 4 . 4 1 . f e h s e l k ' , ' k o l b b v ' , ' k o l b h . a n a l y s i s o f t n f a l p h a ' , ' i n d u c e d d n a s t r a n d b r e a k s a t t h e s i n g l e c e l l l e v e l . a m j p a t h o l . 1 9 9 9 ' , 1 3 9 , 2 5 1 , ' 4 . 4 2 . n e g o e s c u a ' , ' l o r i m i e r p ' , ' l a b a t ' , ' m o l e u r f ' , ' d r o u e t c ' , ' r o b e r t c ' , ' g u i l l e r m e t c c ' , ' e t a l . i n s i t u a p o p t o t i c c e l l l a b e l i n g b y t h e t u n e l m e t h o d ' , ' i m p r o v e m e n t a n d e v a l u a t i o n o n c e l l p r e p a r a t i o n s . j h i s t o c h e m c y t o c h e m . 1 9 9 6 ' , 4 4 , 9 5 9 , ' 6 8 . 4 3 . p o m m i e r y ' , ' p o u r q u i e r p ' , ' u r a s a k i y ' , ' w u j ' , ' l a c o g . t o p o i s o m e r a s e i i n h i b i t o r s ' , ' s e l e c t i v i t y a n d c e l l u l a r r e s i s t a n c e . d r u g r e s i s t a n c e u p d a t e . 1 9 9 9 ' , 2 , 3 0 7 , ' 3 1 8 . 4 4 . c h e n a y ' , ' l i u l f . d n a t o p o i s o m e r a s e s ' , ' e s s e n t i a l e n z y m e s a n d l e t h a l t a r g e t s . a n n u r e v p h a r m a c o l t o x i c o l . 1 9 9 4 ' , 9 4 , 1 9 4 , ' 2 1 8 . 4 5 . b e c k w t ' , ' k h e l i f a t ' , ' k u s u m o t o h ' , ' m o y y ' , ' r o d g e r s q ' , ' w o l v e r t o n j s ' , ' e t a l . n o v e l m e c h a n i s m s o f r e s i s t a n c e t o i n h i b i t o r s o f d n a t o p o i s o m e r a s e s . a d v e n z y m e r e g u l . 1 9 9 7 ' , 3 7 , 1 7 , ' 2 6 . 4 6 . b o s m a n n h b . c a m p t o t h e c i n i n h i b i t s m a c r o m o l e c u l a r s y n t h e s i s i n m a m m a l i a n c e l l s b u t n o t i n i s o l a t e d m i t o c h o n d r i a o r e . c o l i . b i o c h e m b i o p h y s r e s c o m m u n . 1 9 7 0 ' , 4 1 , 1 4 1 2 , ' 2 0 . 4 7 . g a l l o r c ' , ' w h a n g ' , ' p e n g j ' , ' a d a m s o n r h . s t u d i e s o n t h e a n t i t u m o r a c t i v i t y ' , ' m e c h a n i s m o f a c t i o n ' , ' a n d c e l l c y c l e e f f e c t s o f c a m p t o t h e c i n . j n a t c a n c e r i n s t . 1 9 7 1 ' , 4 6 , 7 8 9 , ' 9 5 . 4 8 . h a r t w e l l j l ' , ' a b b o t t b j . a n t i n e o p l a s t i c p r i n c i p l e s i n p l a n t s ' , ' r e c e n t d e v e l o p m e n t s i n t h e f i e l d . a d v p h a r m a c o l c h e m o t h e r . 1 9 6 9 ' , 7 , 1 1 7 , ' 2 0 9 . 4 9 . g o t t l i e b j a ' , ' g u a r i n o a m ' , ' c a l l j b ' , ' o l i v e r i o v t ' , ' b l o c k j b . p r e l i m i n a r y p h a r m a c o l o g i c a n d c l i n i c a l e v a l u a t i o n o f c a m p t o t h e c i n s o d i u m ( n c s ' , ' 1 0 0 8 8 0 ) . c a n c e r c h e m o t h e r r e p . 1 9 7 0 ' , 5 4 , 4 6 1 , ' 7 0 . 5 0 . p a l n c h o n s m ' , ' w u e r z b e r g e r s ' , ' f r y d m a n b ' , ' w i t i a k d t ' , ' h u s t o n p ' , ' c h u r c h d r ' , ' e t a l . β ' , ' l a p a c h o n e m e d i a t e d a p o p t o s i s i n h u m a n p r o m y e l o c y t i c l e u k e m i a ( h l ' , ' 6 0 ) a n d h u m a n p r o s t a t e c a n c e r c e l l s ' , ' a p 5 3 i n d e p e n d e n t r e s p o n s e . c a n c e r r e s . 1 9 9 5 ' , 5 5 , 3 7 0 6 , ' 7 1 1 . 5 1 . t o l i s c ' , ' p e t e r s g j ' , ' f e r r e i r a c g ' , ' p i n e d o h m ' , ' g i a c c o n e g . c e l l c y c l e d i s t u r b a n c e s a n d a p o p t o s i s i n d u c e d b y t o p o t e c a n a n d g e m c i t a b i n e o n h u m a n l u n g c a n c e r c e l l l i n e s . e u r j c a n c e r . 1 9 9 9 ' , 3 5 , 7 9 6 , ' 8 0 7 . 5 2 . r e u b e i g h ' , ' g a r e i s m ' , ' a m s e l g r u b e r w m . c y t o t o x i c i t y e v a l u a t i o n o f m y c o t o x i n s b y a n m t t ' , ' b i o a s s a y . m y c o t o x i n r e s . 1 9 8 7 ' , 3 , 8 5 , ' 9 6 . 5 3 . l i u y ' , ' p e t e r s o n d a ' , ' k i m u r a h ' , ' s c h u b e r t d . m e c h a n i s m o f c e l l u l a r 3 ' , ' ( 4 ' , 5 , ' d i m e t h y l t h i a z o l ' , 2 , ' y l ) ' , 2 , 5 , ' d i p h e n y l t e t r a z o l i u m b r o m i d e ( m t t ) r e d u c t i o n . j n e u r o c h e m . 1 9 9 7 ' , 6 9 , 5 8 1 , ' 9 3 . 5 4 . e b i s u n o s ' , ' i n a g a k i t ' , ' k o h j i m o t o y ' , ' o h k a w a t . t h e c y t o t o x i c e f f e c t o f f e r o x a c i n a n d c i p r o f o x a c i n o n t r a n s i t i o n a l c e l l c a r c i n o m a i n v i t r o . c a n c e r . 1 9 9 7 ' , 8 0 , 2 2 6 3 , ' 7 . 5 5 . g ü r b a y a ' , ' g a r r e l c ' , ' o s m a n m ' , ' r i c h a r d m j ' , ' f a v i e r a ' , ' h i n c a l f . c y t o t o x i c i t y i n c i p r o f o x a c i n ' , ' t r e a t e d h u m a n f i b r o b l a s t c e l l s a n d p r o t e c t i o n b y v i t a m i n e . h u m e x p t o x i c o l . 2 0 0 2 ' , 2 1 , 6 3 5 , ' 4 1 . 5 6 . g ü r b a y a ' , ' g o n t h i e r b ' , ' b a r r e t l ' , ' f a v i e r a ' , ' h i n c a l f . c y t o t o x i c e f f e c t o f c i p r o f o x a c i n i n p r i m a r y c u l t u r e o f r a t a s t r o c y t e s a n d p r o t e c t i o n b y v i t a m i n e . t o x i c o l o g y . 2 0 0 7 ' , 2 2 9 , 5 4 , ' 6 1 . 5 7 . w i j s m a n j a ' , ' d e k a b a n g a ' , ' r i e d e r m j . d i f f e r e n t i a l t o x i c i t y o f r e a c t i v e m e t a b o l i t e s o f c l y n d a m i c i n a n d s u l f o n a m i d e s i n h i v ' , ' i n f e c t e d c e l l s ' , ' i n f u e n c e o f h i v i n f e c t i o n o n c l y n d a m i c i n t o x i c i t y i n v i t r o . j c l i n p h a r m a c o l . 2 0 0 5 ' , 4 5 , 3 4 6 , ' 5 1 . 5 8 . c h e n g y ' , ' p r u s o f f w h . & ' , ' q u o t ' , ' r e l a t i o n s h i p b e t w e e n t h e i n h i b i t i o n c o n s t a n t ( k 1 ) a n d t h e c o n c e n t r a t i o n o f i n h i b i t o r w h i c h c a u s e s 5 0 p e r c e n t i n h i b i t i o n ( i 5 0 ) o f a n e n z y m a t i c r e a c t i o n ' , ' b i o c h e m p h a r m a c o l . 1 9 7 3 ' , 2 2 , 3 0 9 9 , ' 1 0 8 . 5 9 . r i c h a r d r ' , ' n e u b i g r ' , ' m i c h a e l s ' , ' t e r r y k ' , ' a r t h u r c ' , ' n e u b i g r r ' , ' e t a l . i n t e r n a t i o n a l : u n i o n : o f p h a r m a c o l o g y c o m m i t t e e o n r e c e p t o r n o m e n c l a t u r e a n d d r u g c l a s s i f i c a t i o n . x x x v i i i . u p d a t e o n t e r m s a n d s y m b o l s i n q u a n t i t a t i v e p h a r m a c o l o g y . p h a r m a c o l r e v . 2 0 0 3 ' , 5 5 , 5 9 7 , ' 6 0 6 . 6 0 . o s b o m e l s ' , ' h e n l e y r w . v a l u a t i o n o f s a f e r a g r o c h e m ´ s i n s e c t i c i d a l s o a p f e r t h e c o n t r o l o f m i t e s i n t h e i n t e r i o r e n v i r o n m e n t . j f o l i a g e d i g e s t . 2 0 0 0 ' , 5 , 1 0 , ' 1 1 . 6 1 . c i e s i e l s k i m j ' , ' f e n s t e r m a k e r r a . s y n e r g i s t i c c y t o t o x i c i t y ' , ' a p o p t o s i s a n d p r o t e i n l i n k e d d n a b r e a k a g e b y e t o p o s i d e a n d c a m p t o t h e c i n i n h u m a n u 8 7 g l i o m a c e l l s ' , ' d e p e n d e n c e o n t y r o s i n e p h o s p h o r y l a t i o n . j n e u r o o n c o l . 1 9 9 9 ' , 4 1 , 2 2 3 , ' 3 4 . 6 2 . h u s s a i n s m ' , ' h e s s k l ' , ' g e a r h a r t j m ' , ' g e i s s k t ' , ' s c h l a g e r j j . i n v i t r o t o x i c i t y o f n a n o p a r t i c l e s i n b r l 3 a r a t l i v e r c e l l s . t o x i c o l o g y i n v i t r o . 2 0 0 5 ' , 1 9 , 9 7 5 , ' 8 3 . 6 3 . l i n w s ' , ' h u a n g y w ' , ' z h o u x d ' , ' m a y f . i n v i t r o t o x i c i t y o f s i l i c a n a n o p a r t i c l e s i n h u m a n l u n g c a n c e r c e l l s . t o x i c o l o g y a n d a p p l i e d p h a r m a c o l o g y . 2 0 0 6 ' , 2 1 7 , 2 5 2 , ' 9 . 6 4 . t s u j i m o t o y . a p o p t o s i s a n d n e c r o s i s ' , ' i n t r a c e l l u l a r a t p l e v e l a s a d e t e r m i n a n t f o r c e l l d e a t h m o d e s . c e l l d e a t h d i f f e r . 1 9 9 7 ' , 4 , 4 2 9 , ' 3 4 . 6 5 . n i c o t e r a p ' , ' l e i s t m ' , ' f e r r a n d o ' , ' m a y e . i n t r a c e l l u l a r a t p ' , ' a s w i t c h i n t h e d e c i s i o n b e t w e e n a p o p t o s i s a n d n e c r o s i s . t o x i c o l l e t t . 1 9 9 8 ' , 1 0 2 , 1 3 9 , ' 4 2 . 6 6 . f o r m i g l i l ' , ' p a p u c c i l ' , ' t a n i a ' , ' s c h i a v o n e n ' , ' t e m p e s t i n i a ' , ' o r l a n d i n i g e ' , ' e t a l . a p o n e c r o s i s ' , ' m o r p h o l o g i c a l a n d b i o c h e m i c a l e x p l o r a t i o n o f a s y n c r e t i c p r o c e s s o f c e l l d e a t h s h a r i n g a p o p t o s i s a n d n e c r o s i s . j c e l l p h y s i o l . 2 0 0 0 ' , 1 8 2 , 4 1 , ' 9 . 6 7 . f i n s t a d h s ' , ' k o l s e t s o ' , ' h o l m e j a ' , ' w i g e r r ' , ' f a r r a n t s a k ' , ' b l o m h o f f r ' , ' e t a l . e f f e c t o f n ' , ' 3 a n d n ' , ' 6 f a t t y a c i d s o n p r o l i f e r a t i o n a n d d i f f e r e n t i a t i o n o f p r o m y e l o c y t i c l e u k e m i c h l ' , ' 6 0 c e l l s . b l o o d . 1 9 9 4 ' , 8 4 , 3 7 9 9 , ' 8 0 9 . 6 8 . k e r r j f r ' , ' w y l l i e a h ' , ' c u r r i r e a r . a p o p t o s i s ' , ' a b a s i c b i o l o g i c a l p h e n o m e n o n w i t h w i d e r a n g i n g i m p l i c a t i o n s i n t i s s u e k i n e t i c s . b r j c a n c e r . 1 9 7 2 ' , 2 6 , ' 2 3 9 5 7 . 6 9 . g r a z i a n i g ' , ' s z a b o c . c l i n i c a l p e r s p e c t i v e s o f p a r p i n h i b i t o r s . p h a r m a c o l r e s . 2 0 0 5 ' , 5 2 , 1 0 9 , ' 1 8 . 7 0 . h o n d a o ' , ' k u r o d a m ' , ' j o j a i ' , ' a s a u m i j ' , ' t a k e d a y ' , ' a k a k i s ' , ' e t a l . a s s e s s m e n t o f s e c o n d a r y n e c r o s i s o f j u r k a t c e l l s u s i n g a n e w m i c r o s c o p i c s y s t e m a n d d o u b l e s t a i n i n g m e t h o d w i t h a n n e x i n v a n d p r o p i d i u m i o d i d e . i n t j o n c o l . 2 0 0 0 ' , 1 6 , 2 8 3 , ' 8 . 7 1 . i n g a l l s c p ' , ' b a r n e s w s ' , ' s m i t h s b . i n t e r a c t i o n b e t w e e n c l e n b u t e r o l a n d r u n t r a i n i n g ' , ' e f f e c t s o n e x e r c i s e p e r f o r m a n c e a n d m l c i s o f o r m c o n t e n t . j a p p l p h y s i o l . 1 9 9 6 ' , 8 0 , 7 9 5 , ' 8 0 1 . 7 2 . l e i s t m ' , ' j a a t t e l a m . & ' , ' q u o t ' , ' f o u r d e a t h s a n d a f u n e r a l ' , ' f r o m c a s p a s e s t o a l t e r n a t i v e m e c h a n i s m s . & ' , ' q u o t ' , ' n a t r e v m o l c e l l b i o l . 2 0 0 1 ' , 2 , 5 8 9 , ' 9 8 . 7 3 . b l a n k m ' , ' s h i l o h y . p r o g r a m s f o r c e l l d e a t h ' , ' a p o p t o s i s i s o n l y o n e w a y t o g o . c e l l c y c l e . 2 0 0 7 ' , 6 , 6 8 6 , ' 9 5 . 7 4 . b a u m a n n s ' , ' k r u e g e r a ' , ' k i r c h h o f f s ' , ' k r a m m e r p h . r e g u l a t i o n o f t c e l l a p o p t o s i s d u r i n g t h e i m m u n e r e s p o n s e . c u r r e n t m o l e c u l a r m e d i c i n e . 2 0 0 2 ' , 2 , 2 5 7 , ' 7 2 . 7 5 . n a g a t a s . a p o p t o t i c d n a f r a g m e n t a t i o n . e x p c e l l r e s . 2 0 0 0 ' , 2 5 6 , 1 2 , ' 1 8 . 7 6 . w y l l i e a h . g l u c o r c o r t i c o i d ' , ' i n d u c e d t h y m o c y t e a p o p t o s i s i s a s s o c i a t e d w i t h e n d o g e n o u s e n d o n u c l e a s e a c t i v a t i o n . n a t u r e . 1 9 8 0 ' , 2 8 4 , 5 5 5 , ' 6 . ' ]

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